Saturday, January 14, 2012

DOES LIGHT AND TEMPERATURE RENDER KANAMYCIN INEFFECTIVE?

I infused my LB agar plates with a final concentration of 30 ug/mL Kanamycin. I transformed some presumed competent E. coli with a vector containing Kanamycin resistance then spread them on one with ampicillin and one on the aforementioned kanamycin plates, and grew overnight at 37 C. the negative control didnt grow but the kanamycin plate did. THE MAIN QUESTION IS, DOES LIGHT AND TEMPERATURE RENDER KANAMYCIN INEFFECTIVE?

0 comments:

Post a Comment